Control:Article Title: A stress sensor, IRE1α, is required for bacterial-exotoxin-induced interleukin-1β production in tissue-resident macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IRE1a cell signaling technology Cat: 3294; RRID:AB_823545 anti-IRE1a Mybiosource Cat: MBS421918 anti-Caspase-1 p10 Santa Cruz Biotechnology Cat: sc-514; RRID:AB_2068895 anti-CD16/32 TONBO biosciences Cat: 70-0161-M001 anti-mouse F4/80 BIO-RAD Cat: MCA497B; RRID:AB_322046 V450-conjugated anti-CD11b BD Biosciences Cat:560456; RRID:AB_1645267 anti-XBP-1S-PE BD Biosciences Cat: 562642; RRID:AB_2687882 control isotype mouse IgG1 PE Biolegend Cat: 400112; RRID:AB_2847829 normal rabbit IgG Cell Signaling Technology Cat: 3900; RRID:AB_1550038 Alexa 546-Labelled Goat anti rabbit IgG Invitrogen Cat: A11010; RRID:AB_2534077 anti-b-actin Ab (C4) Santa Cruz Biotechnology Cat: sc-47778; RRID:AB_626632 anti-CT abcam Cat: ab123129; RRID:AB_10971380 anti-CT Mybiosource Cat: MBS 560685; RRID:AB_10884943 biotinylated anti-IL-1b R&D Systems Cat: BAF401; RRID:AB_356450 anti-GSDMD abcam Cat: ab209845; RRID:AB_2783550 Anti-ganglioside GM1 Bioss Cat:bs-2367R-A647 anti-ASC Cell signaling technology Cat: 67824S; RRID:AB_2799736 Anti-KDEL Enzo Life Sciences Cat: ADI-SPA-827-D; RRID; AB_2039327 anti-mouse IgG-HRP GE Healthcare Cat: NA931V anti-rabbit IgG-HRP GE Healthcare Cat: NA934V Anti-goat IgG-HRP Santacruz Cat: sc-2020 Streptavidin-HRP Conjugate Merck Cat: RPN1231 Chemicals, peptides, and recombinant proteins LPS O55:B5 Sigma-Aldrich Cat: L2637 LPS O111:B4 Sigma-Aldrich Cat: L3012 CTB List Biological Laboratories Cat: 103B CT List Biological Laboratories Cat: 100B ATP InvivoGen Cat: tlrl-atp TcdB R&D systems Cat: 6246-GT-020 Tunicamycin Calbiochem (Merck) Cat: 654380-10MGCN 4m8c Cayman Chemical Cat: 14003-96-4 GSK2850163 Sigma-Aldrich Cat: SML-1684-5MG GSK2606414 Tocris Bioscience Cat: 5107 MCC950 Selleck Cat: S7809 Dimethyl sulfoxide Nacalai Tesque Cat: 13408-64 protease inhibitor cocktail Roche Cat: 11836153001 FITC-CTB Sigma-Aldrich Cat: C1655 normal goat serum Vector Laboratories Inc. Cat: S-1000 Vectashiled Mounting Medium with DAPI Vector Laboratories Inc. Cat: H1200 PerCP-Cy5.5-conjugated streptavidin BD Biosciences Cat: 551419; RRID:AB_2868934 Critical commercial assays Mouse IL-1 beta/IL-1F2 Quantikine ELISA kit R&D systems Cat: SMLB00C Mouse IL-6 DuoSet ELISA R&D systems Cat: DY406 (Continued on next page) 16 Cell Reports 43, 113981, April 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Membrane Protein Extraction Kit Thermo Fisher Scientific Cat: 89842 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set Invitrogen Cat: 00-5523-00 LIVE/DEAD Fixable Dead Cell Aqua Stain Kit Invitrogen Cat: L34966 CytoSelect LDH Cytotoxicity Assay Kit Cell Biolabs Cat: CBA-241 Deposited data Mouse RPMs RNA-seq This paper GEO: GSE228915 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan N/A b1,4-N-acetylgalactosaminyltransferase (B4galnt1)-deficient mice Previous study Takamiya et al.38,39 apoptosis-associated speck-like protein containing CARD (ASC)-deficient mice Previous study Mariathasan et al.40–42 NLRP3-deficient mice Previous study Mariathasan et al.40–42 Pyrin-deficient mice Previous study Orimo et al.8 Floxed IRE1a (Ern1fl/fl) mice Previous study Iwawaki et al.25 Lysozyme 2-Cre (Lyz2+/cre) mice Previous study Clausen et al.26 Oligonucleotides sXbp1 sense: 50-AAGAACACGCTTGGGAATGG-30 FASMAC Márquez et al.43 sXbp1 antisense: 50-CTGCACCTGCTGCGGAC-30 FASMAC Márquez et al.43 Ddit3 sense: 50-CCCAGGAAACGAAGAGGAAG-30 FASMAC Kim et al.44 Ddit3 antisense: 50-AGTGCAGTGCAGGGTCACAT-30 FASMAC Kim et al.44 Software and algorithms FlowJo Version 8.8.7 TreeStar The DAVID Bioinfomatics database Previous study Huang et al.45 Kyoto Encyclopedia of Genes and Genomes (KEGG) Previous study Ogata et al.46 The web tool ClustVis Previous study Metsalu et al.47 The web tool BioJupies Previous study Torre et al.48 ImageJ 1.50i Schneider et al.49 http://imagej.nih.gov/ij Other CELLview cell culture dish Greiner Bio-One International Cat: 627975 Cell-Tak solution Corning Cat: 354240 polyvinylidene difluoride membranes Bio-Rad Cat: 1620177 nitrocellulose membrane Bio-Rad Cat: 1620146 protease inhibitor cocktail Roche Cat: 11836153001
Recombinant:Article Title: A stress sensor, IRE1α, is required for bacterial-exotoxin-induced interleukin-1β production in tissue-resident macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IRE1a cell signaling technology Cat: 3294; RRID:AB_823545 anti-IRE1a Mybiosource Cat: MBS421918 anti-Caspase-1 p10 Santa Cruz Biotechnology Cat: sc-514; RRID:AB_2068895 anti-CD16/32 TONBO biosciences Cat: 70-0161-M001 anti-mouse F4/80 BIO-RAD Cat: MCA497B; RRID:AB_322046 V450-conjugated anti-CD11b BD Biosciences Cat:560456; RRID:AB_1645267 anti-XBP-1S-PE BD Biosciences Cat: 562642; RRID:AB_2687882 control isotype mouse IgG1 PE Biolegend Cat: 400112; RRID:AB_2847829 normal rabbit IgG Cell Signaling Technology Cat: 3900; RRID:AB_1550038 Alexa 546-Labelled Goat anti rabbit IgG Invitrogen Cat: A11010; RRID:AB_2534077 anti-b-actin Ab (C4) Santa Cruz Biotechnology Cat: sc-47778; RRID:AB_626632 anti-CT abcam Cat: ab123129; RRID:AB_10971380 anti-CT Mybiosource Cat: MBS 560685; RRID:AB_10884943 biotinylated anti-IL-1b R&D Systems Cat: BAF401; RRID:AB_356450 anti-GSDMD abcam Cat: ab209845; RRID:AB_2783550 Anti-ganglioside GM1 Bioss Cat:bs-2367R-A647 anti-ASC Cell signaling technology Cat: 67824S; RRID:AB_2799736 Anti-KDEL Enzo Life Sciences Cat: ADI-SPA-827-D; RRID; AB_2039327 anti-mouse IgG-HRP GE Healthcare Cat: NA931V anti-rabbit IgG-HRP GE Healthcare Cat: NA934V Anti-goat IgG-HRP Santacruz Cat: sc-2020 Streptavidin-HRP Conjugate Merck Cat: RPN1231 Chemicals, peptides, and recombinant proteins LPS O55:B5 Sigma-Aldrich Cat: L2637 LPS O111:B4 Sigma-Aldrich Cat: L3012 CTB List Biological Laboratories Cat: 103B CT List Biological Laboratories Cat: 100B ATP InvivoGen Cat: tlrl-atp TcdB R&D systems Cat: 6246-GT-020 Tunicamycin Calbiochem (Merck) Cat: 654380-10MGCN 4m8c Cayman Chemical Cat: 14003-96-4 GSK2850163 Sigma-Aldrich Cat: SML-1684-5MG GSK2606414 Tocris Bioscience Cat: 5107 MCC950 Selleck Cat: S7809 Dimethyl sulfoxide Nacalai Tesque Cat: 13408-64 protease inhibitor cocktail Roche Cat: 11836153001 FITC-CTB Sigma-Aldrich Cat: C1655 normal goat serum Vector Laboratories Inc. Cat: S-1000 Vectashiled Mounting Medium with DAPI Vector Laboratories Inc. Cat: H1200 PerCP-Cy5.5-conjugated streptavidin BD Biosciences Cat: 551419; RRID:AB_2868934 Critical commercial assays Mouse IL-1 beta/IL-1F2 Quantikine ELISA kit R&D systems Cat: SMLB00C Mouse IL-6 DuoSet ELISA R&D systems Cat: DY406 (Continued on next page) 16 Cell Reports 43, 113981, April 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Membrane Protein Extraction Kit Thermo Fisher Scientific Cat: 89842 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set Invitrogen Cat: 00-5523-00 LIVE/DEAD Fixable Dead Cell Aqua Stain Kit Invitrogen Cat: L34966 CytoSelect LDH Cytotoxicity Assay Kit Cell Biolabs Cat: CBA-241 Deposited data Mouse RPMs RNA-seq This paper GEO: GSE228915 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan N/A b1,4-N-acetylgalactosaminyltransferase (B4galnt1)-deficient mice Previous study Takamiya et al.38,39 apoptosis-associated speck-like protein containing CARD (ASC)-deficient mice Previous study Mariathasan et al.40–42 NLRP3-deficient mice Previous study Mariathasan et al.40–42 Pyrin-deficient mice Previous study Orimo et al.8 Floxed IRE1a (Ern1fl/fl) mice Previous study Iwawaki et al.25 Lysozyme 2-Cre (Lyz2+/cre) mice Previous study Clausen et al.26 Oligonucleotides sXbp1 sense: 50-AAGAACACGCTTGGGAATGG-30 FASMAC Márquez et al.43 sXbp1 antisense: 50-CTGCACCTGCTGCGGAC-30 FASMAC Márquez et al.43 Ddit3 sense: 50-CCCAGGAAACGAAGAGGAAG-30 FASMAC Kim et al.44 Ddit3 antisense: 50-AGTGCAGTGCAGGGTCACAT-30 FASMAC Kim et al.44 Software and algorithms FlowJo Version 8.8.7 TreeStar The DAVID Bioinfomatics database Previous study Huang et al.45 Kyoto Encyclopedia of Genes and Genomes (KEGG) Previous study Ogata et al.46 The web tool ClustVis Previous study Metsalu et al.47 The web tool BioJupies Previous study Torre et al.48 ImageJ 1.50i Schneider et al.49 http://imagej.nih.gov/ij Other CELLview cell culture dish Greiner Bio-One International Cat: 627975 Cell-Tak solution Corning Cat: 354240 polyvinylidene difluoride membranes Bio-Rad Cat: 1620177 nitrocellulose membrane Bio-Rad Cat: 1620146 protease inhibitor cocktail Roche Cat: 11836153001
Protease Inhibitor:Article Title: A stress sensor, IRE1α, is required for bacterial-exotoxin-induced interleukin-1β production in tissue-resident macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IRE1a cell signaling technology Cat: 3294; RRID:AB_823545 anti-IRE1a Mybiosource Cat: MBS421918 anti-Caspase-1 p10 Santa Cruz Biotechnology Cat: sc-514; RRID:AB_2068895 anti-CD16/32 TONBO biosciences Cat: 70-0161-M001 anti-mouse F4/80 BIO-RAD Cat: MCA497B; RRID:AB_322046 V450-conjugated anti-CD11b BD Biosciences Cat:560456; RRID:AB_1645267 anti-XBP-1S-PE BD Biosciences Cat: 562642; RRID:AB_2687882 control isotype mouse IgG1 PE Biolegend Cat: 400112; RRID:AB_2847829 normal rabbit IgG Cell Signaling Technology Cat: 3900; RRID:AB_1550038 Alexa 546-Labelled Goat anti rabbit IgG Invitrogen Cat: A11010; RRID:AB_2534077 anti-b-actin Ab (C4) Santa Cruz Biotechnology Cat: sc-47778; RRID:AB_626632 anti-CT abcam Cat: ab123129; RRID:AB_10971380 anti-CT Mybiosource Cat: MBS 560685; RRID:AB_10884943 biotinylated anti-IL-1b R&D Systems Cat: BAF401; RRID:AB_356450 anti-GSDMD abcam Cat: ab209845; RRID:AB_2783550 Anti-ganglioside GM1 Bioss Cat:bs-2367R-A647 anti-ASC Cell signaling technology Cat: 67824S; RRID:AB_2799736 Anti-KDEL Enzo Life Sciences Cat: ADI-SPA-827-D; RRID; AB_2039327 anti-mouse IgG-HRP GE Healthcare Cat: NA931V anti-rabbit IgG-HRP GE Healthcare Cat: NA934V Anti-goat IgG-HRP Santacruz Cat: sc-2020 Streptavidin-HRP Conjugate Merck Cat: RPN1231 Chemicals, peptides, and recombinant proteins LPS O55:B5 Sigma-Aldrich Cat: L2637 LPS O111:B4 Sigma-Aldrich Cat: L3012 CTB List Biological Laboratories Cat: 103B CT List Biological Laboratories Cat: 100B ATP InvivoGen Cat: tlrl-atp TcdB R&D systems Cat: 6246-GT-020 Tunicamycin Calbiochem (Merck) Cat: 654380-10MGCN 4m8c Cayman Chemical Cat: 14003-96-4 GSK2850163 Sigma-Aldrich Cat: SML-1684-5MG GSK2606414 Tocris Bioscience Cat: 5107 MCC950 Selleck Cat: S7809 Dimethyl sulfoxide Nacalai Tesque Cat: 13408-64 protease inhibitor cocktail Roche Cat: 11836153001 FITC-CTB Sigma-Aldrich Cat: C1655 normal goat serum Vector Laboratories Inc. Cat: S-1000 Vectashiled Mounting Medium with DAPI Vector Laboratories Inc. Cat: H1200 PerCP-Cy5.5-conjugated streptavidin BD Biosciences Cat: 551419; RRID:AB_2868934 Critical commercial assays Mouse IL-1 beta/IL-1F2 Quantikine ELISA kit R&D systems Cat: SMLB00C Mouse IL-6 DuoSet ELISA R&D systems Cat: DY406 (Continued on next page) 16 Cell Reports 43, 113981, April 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Membrane Protein Extraction Kit Thermo Fisher Scientific Cat: 89842 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set Invitrogen Cat: 00-5523-00 LIVE/DEAD Fixable Dead Cell Aqua Stain Kit Invitrogen Cat: L34966 CytoSelect LDH Cytotoxicity Assay Kit Cell Biolabs Cat: CBA-241 Deposited data Mouse RPMs RNA-seq This paper GEO: GSE228915 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan N/A b1,4-N-acetylgalactosaminyltransferase (B4galnt1)-deficient mice Previous study Takamiya et al.38,39 apoptosis-associated speck-like protein containing CARD (ASC)-deficient mice Previous study Mariathasan et al.40–42 NLRP3-deficient mice Previous study Mariathasan et al.40–42 Pyrin-deficient mice Previous study Orimo et al.8 Floxed IRE1a (Ern1fl/fl) mice Previous study Iwawaki et al.25 Lysozyme 2-Cre (Lyz2+/cre) mice Previous study Clausen et al.26 Oligonucleotides sXbp1 sense: 50-AAGAACACGCTTGGGAATGG-30 FASMAC Márquez et al.43 sXbp1 antisense: 50-CTGCACCTGCTGCGGAC-30 FASMAC Márquez et al.43 Ddit3 sense: 50-CCCAGGAAACGAAGAGGAAG-30 FASMAC Kim et al.44 Ddit3 antisense: 50-AGTGCAGTGCAGGGTCACAT-30 FASMAC Kim et al.44 Software and algorithms FlowJo Version 8.8.7 TreeStar The DAVID Bioinfomatics database Previous study Huang et al.45 Kyoto Encyclopedia of Genes and Genomes (KEGG) Previous study Ogata et al.46 The web tool ClustVis Previous study Metsalu et al.47 The web tool BioJupies Previous study Torre et al.48 ImageJ 1.50i Schneider et al.49 http://imagej.nih.gov/ij Other CELLview cell culture dish Greiner Bio-One International Cat: 627975 Cell-Tak solution Corning Cat: 354240 polyvinylidene difluoride membranes Bio-Rad Cat: 1620177 nitrocellulose membrane Bio-Rad Cat: 1620146 protease inhibitor cocktail Roche Cat: 11836153001
Enzyme-linked Immunosorbent Assay:Article Title: A stress sensor, IRE1α, is required for bacterial-exotoxin-induced interleukin-1β production in tissue-resident macrophages.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-IRE1a cell signaling technology Cat: 3294; RRID:AB_823545 anti-IRE1a Mybiosource Cat: MBS421918 anti-Caspase-1 p10 Santa Cruz Biotechnology Cat: sc-514; RRID:AB_2068895 anti-CD16/32 TONBO biosciences Cat: 70-0161-M001 anti-mouse F4/80 BIO-RAD Cat: MCA497B; RRID:AB_322046 V450-conjugated anti-CD11b BD Biosciences Cat:560456; RRID:AB_1645267 anti-XBP-1S-PE BD Biosciences Cat: 562642; RRID:AB_2687882 control isotype mouse IgG1 PE Biolegend Cat: 400112; RRID:AB_2847829 normal rabbit IgG Cell Signaling Technology Cat: 3900; RRID:AB_1550038 Alexa 546-Labelled Goat anti rabbit IgG Invitrogen Cat: A11010; RRID:AB_2534077 anti-b-actin Ab (C4) Santa Cruz Biotechnology Cat: sc-47778; RRID:AB_626632 anti-CT abcam Cat: ab123129; RRID:AB_10971380 anti-CT Mybiosource Cat: MBS 560685; RRID:AB_10884943 biotinylated anti-IL-1b R&D Systems Cat: BAF401; RRID:AB_356450 anti-GSDMD abcam Cat: ab209845; RRID:AB_2783550 Anti-ganglioside GM1 Bioss Cat:bs-2367R-A647 anti-ASC Cell signaling technology Cat: 67824S; RRID:AB_2799736 Anti-KDEL Enzo Life Sciences Cat: ADI-SPA-827-D; RRID; AB_2039327 anti-mouse IgG-HRP GE Healthcare Cat: NA931V anti-rabbit IgG-HRP GE Healthcare Cat: NA934V Anti-goat IgG-HRP Santacruz Cat: sc-2020 Streptavidin-HRP Conjugate Merck Cat: RPN1231 Chemicals, peptides, and recombinant proteins LPS O55:B5 Sigma-Aldrich Cat: L2637 LPS O111:B4 Sigma-Aldrich Cat: L3012 CTB List Biological Laboratories Cat: 103B CT List Biological Laboratories Cat: 100B ATP InvivoGen Cat: tlrl-atp TcdB R&D systems Cat: 6246-GT-020 Tunicamycin Calbiochem (Merck) Cat: 654380-10MGCN 4m8c Cayman Chemical Cat: 14003-96-4 GSK2850163 Sigma-Aldrich Cat: SML-1684-5MG GSK2606414 Tocris Bioscience Cat: 5107 MCC950 Selleck Cat: S7809 Dimethyl sulfoxide Nacalai Tesque Cat: 13408-64 protease inhibitor cocktail Roche Cat: 11836153001 FITC-CTB Sigma-Aldrich Cat: C1655 normal goat serum Vector Laboratories Inc. Cat: S-1000 Vectashiled Mounting Medium with DAPI Vector Laboratories Inc. Cat: H1200 PerCP-Cy5.5-conjugated streptavidin BD Biosciences Cat: 551419; RRID:AB_2868934 Critical commercial assays Mouse IL-1 beta/IL-1F2 Quantikine ELISA kit R&D systems Cat: SMLB00C Mouse IL-6 DuoSet ELISA R&D systems Cat: DY406 (Continued on next page) 16 Cell Reports 43, 113981, April 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Membrane Protein Extraction Kit Thermo Fisher Scientific Cat: 89842 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set Invitrogen Cat: 00-5523-00 LIVE/DEAD Fixable Dead Cell Aqua Stain Kit Invitrogen Cat: L34966 CytoSelect LDH Cytotoxicity Assay Kit Cell Biolabs Cat: CBA-241 Deposited data Mouse RPMs RNA-seq This paper GEO: GSE228915 Experimental models: Organisms/strains C57BL/6 mice CLEA Japan N/A b1,4-N-acetylgalactosaminyltransferase (B4galnt1)-deficient mice Previous study Takamiya et al.38,39 apoptosis-associated speck-like protein containing CARD (ASC)-deficient mice Previous study Mariathasan et al.40–42 NLRP3-deficient mice Previous study Mariathasan et al.40–42 Pyrin-deficient mice Previous study Orimo et al.8 Floxed IRE1a (Ern1fl/fl) mice Previous study Iwawaki et al.25 Lysozyme 2-Cre (Lyz2+/cre) mice Previous study Clausen et al.26 Oligonucleotides sXbp1 sense: 50-AAGAACACGCTTGGGAATGG-30 FASMAC Márquez et al.43 sXbp1 antisense: 50-CTGCACCTGCTGCGGAC-30 FASMAC Márquez et al.43 Ddit3 sense: 50-CCCAGGAAACGAAGAGGAAG-30 FASMAC Kim et al.44 Ddit3 antisense: 50-AGTGCAGTGCAGGGTCACAT-30 FASMAC Kim et al.44 Software and algorithms FlowJo Version 8.8.7 TreeStar The DAVID Bioinfomatics database Previous study Huang et al.45 Kyoto Encyclopedia of Genes and Genomes (KEGG) Previous study Ogata et al.46 The web tool ClustVis Previous study Metsalu et al.47 The web tool BioJupies Previous study Torre et al.48 ImageJ 1.50i Schneider et al.49 http://imagej.nih.gov/ij Other CELLview cell culture dish Greiner Bio-One International Cat: 627975 Cell-Tak solution Corning Cat: 354240 polyvinylidene difluoride membranes Bio-Rad Cat: 1620177 nitrocellulose membrane Bio-Rad Cat: 1620146 protease inhibitor cocktail Roche Cat: 11836153001
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